Heat shock protein 27 and 70 play roles in neurotoxicity caused by manganese in rats

AIM To explore the role of Heat shock protein 27 and 70 (Hsp27, Hsp70) in neurotoxicity caused by manganese (Mn²⁺) in rats. METHODS 50 SD rats, adult and healthy, are divided into 5 groups at random: normal saline (A), Mn²⁺ 2.5mg/Kg (B), Mn²⁺ 5mg/Kg (C)�AMn²⁺ 10mg/Kg (D)�AMn²⁺ 20mg/Kg (E). They are exposed to Mn²⁺ by intraperitioneal injection at the dose of 0.5ml/d, 30d. After exposure, we detected the concentration of brain Mn²⁺ using ICP-AES (inductively coupled plasma-atomic emission spectrometry) .The mRNA expressions of Hsp27 and Hsp70 in brains in rats were measured by real-time fluorescence quantitative PCR (QT-PCR). RESULTS The concentration of brain Mn²⁺ increases significantly in groups of B, C, D, and E compared with group A (P<0.01). QT-PCR shows a 2.00�A5.51�A8.78and6.61 fold increase of Hsp27 mRNA expression, 1.68, 10.27, 22.88, and 2.20 fold increase of Hsp70 mRNA expression in group B, C, D, and E respectively compared with group A. CONCLUSION Heat shock protein 27and 70 can protect brain of rats from injury in some extent.

Key words: manganese, Hsp27; Hsp70, real-time fluorescence quantitative PCR

Grants support: Natural Science Foundation of China (NSFC: 30700657) and Fund for Foster Excellent Talents of Beijing (20071D0501800250)