Serotype identification and antibiotic resistance profiles of salmonella spp. isolated from broiler carcasses

 Abstract
                  The objectives of this study were, to determine the serotype distribution of 32 Salmonella strains isolated from broiler carcasses in Ankara, Turkey, and to find out the antibiotic resistance profiles of the isolates against 10 different antibiotics. It was found that, out of 32 Salmonella isolates, 22 (68.8 %), 5 (15.6 %), 3 (9.4 %), and 2 (6.3 %) were serotyped as Enteritidis, Virchow, Typhimurium and Hadar, respectively, according to Kauffmann-White scheme using lam agglutination and serum neutralization. By disc diffusion test, 20 (62.5 %) Salmonella isolates were resistant to nalidixic acid while, 4 (12.5 %) to cephalothin, 2 (6.3 %) to streptomycin, and 2 (6.3 %) to tetracycline. Among 4 (12.5 %) strains which displayed multi-drug resistance, 2 (6.3 %) were resistant to tetracycline, streptomycin and nalidixic acid; 2 (6.3 %) were resistant to cephalothin and nalidixic acid. All of the 2 S. Hadar were found to be resistant to tetracycline, streptomycin and nalidixic acid. Fifteen (68.1 %) of 22 S. Enteritidis, 1 (33.3 %) of 3 S. Typhimurium and 2 (40.0 %) of 5 S. Virchow showed resistance to nalidixic acid. In addition, 2 (9.0 %), 1 (33.3 %) and 1 (20.0 %) of S. Enteritidis, S. Typhimurium and S. Virchow showed resistance to cephalotin, respectively. Out of 32 Salmonella strains, predominant serotype was S. Enteritidis (22/32) with a high (15/22) frequency of nalidixic acid resistance and S. Hadar was found to be the most resistant serotype to antibiotics. The results indicated that, the incidence of antibiotic resistant Salmonella strains in broiler carcasses is also a public health concern in Turkey, in compliance worldwide.